In colaboration with

Methodology

This project proposal builds on an existing project Plant-associated microorganisms for improved grain production (CSP295) funded by the GRDC due to finish in June 2002.  This laboratory-based project is studying the impact of bacteria located inside the plant (endophytes) on plant growth and disease resistance.   

Endophytes have an advantage over other bacteria in the soil because they are protected within the plant from adverse environmental conditions (lack of moisture, adverse temperatures and competition from other soil microorganisms) and have a ready supply of food from the plant. The group of bacteria targeted (actinomycetes) is known to produce both antifungal and growth promoting compounds.

Cereal plants were surveyed across the wheat belt and nearly 300 actinomycetes were isolated from within plant roots.  (58 isolates were obtained from Valema Farms, Corrigin).  These bacterial isolates are currently being tested for the ability to promote plant growth and suppress disease in laboratory and glasshouse trials.  So far, of the 61 isolates tested in the glasshouse, 53% resulted in significant plant growth promotion (shoot and /or root mass) up to 37% (P<0.05).  4% resulted in significant suppression of Rhizoctonia up to 35% (P<0.05).  Collaborators (Assoc Prof Chris Franco at Flinders University SA) have measured significant plant growth promotion and disease suppression in similar glasshouse trials in South Australia.  They have already commenced field trials.

In this proposal we are seeking funding to determine if endophytes, that resulted in plant growth promotion and/or disease suppression in the glasshouse trials, also provide yield increases under field conditions.